Pharmaceutical Methods
Publishing Quality Research & Reviews
Pharmaceutical Methods
Publishing Quality Research & Reviews
Author(s): Madhuri Ajay Hinge, Jigar Arjunbhai Patel, Rajvi J. Mahida
Introduction: Absorbance ratio spectrophotometric method and high performance liquid chromatographic method were developed for the determination of the Amlodipine Besylate and Nebivolol Hydrochloride in tablet dosage form. Methods: Absorbance ratio spectrophotometric method and high performance liquid chromatographic method were developed for the determination of the Amlodipine Besylate and Nebivolol Hydrochloride in tablet dosage form. A simple liquid chromatographic assay has been developed for the determination of Amlodipine Besylate and Nebivolol Hydrochloride. A C18 (250×4.6 mm, 5 μ) column was used with a mobile phase consisting of Water: Acetonitrile ( pH adjusted to 3.5 with ortho phosphoric acid) at a flow rate of 1.0 ml min-1. Quantitation was achieved with UV detection at 268 nm based on the peak height ratios. Results: Calibration curves were linear in the range of 10–30 μg/ml for Amlodipine Besylate and 10-30 μg/ml for Nebivolol Hydrochloride in absorbance ratio method. Correlation coefficient found to be close to 0.9995 for both the drugs. Accuracy for both the drugs was in the range of 99-101%. Beer’s law was obeyed in a concentration range of 10-30 mg ml-1 for Amlodipine Besylate and 10-30 mg ml-1 for Nebivolol Hydrochloride and the regression line equation was derived with a correlation coefficient of 0.9999 and 0.9998 for Amlodipine Besylate and Nebivolol Hydrochloride respectively. Discussion: The proposed procedures were successfully applied to the determination of Amlodipine Besylate and Nebivolol Hydrochloride in bulk and tablet form, with high percentage of recovery, good accuracy and precision.
