Spectrophotometric and High Performance Liquid Chromatographic Determination (HPLC) of Triprolidine and Pseudoephedrine Hydrochloride in Tablet Dosage Form

Abstract

Author(s): Madhuri Hinge, K. R. Patel and R. J. Mahida

First order derivative spectrophotometric method and high performance liquid chromatographic method were developed for the determination of Triprolidine and Pseudoephedrine Hydrochloride in tablet dosage form. In UV-Spectrophotometric method, estimation of Triprolidine and Pseudoephedrine Hydrochloride was carried out at the wavelength selected 246.20 nm and 263.50 nm for First order Derivative method. Calibration curves were linear in the range of 2-10 μg ml^-1 for Triprolidine and 48- 240 μg ml^-1 for Pseudoephedrine Hydrochloride in derivative method. Correlation coefficient found to be close to 0.9950 for both the drugs. Accuracy for both the drugs was in the range of 99-101.5%. A simple liquid chromatographic assay has been developed for the determination of Triprolidine and Pseudoephedrine Hydrochloride. A C₁₈ (250×4.6 mm, 5 μm) column was used with a mobile phase consisting of Methanol: Water (80: 20 v/v) (pH adjusted to 3.0 with ortho phosphoric acid) at a flow rate of 1.0 ml min-1. Quantitation was achieved with UV detection at 246.20 nm based on the peak height ratios. Beer’s law was obeyed in a concentration range of 5-25 μg ml^-1 for Triprolidine and 120-600 μg ml^-1 for Pseudoephedrine Hydrochloride and the regression line equation was derived with a correlation coefficient of 0.9999 and 0.9998 for Triprolidine and Pseudoephedrine Hydrochloride. The proposed procedures were successfully applied to the determination of Triprolidine and Pseudoephedrine Hydrochloride in bulk and tablet form, with high percentage of recovery, good accuracy and precision.

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