Pharmaceutical Methods
Publishing Quality Research & Reviews
Pharmaceutical Methods
Publishing Quality Research & Reviews
Author(s): Bhusarapu Satya Prasad*, Sekharan Jaya Kumari
The validated protein precipitation method was applied for estimation of Eravacycline dihydrochloride in human plasma with Rolitetracycline hydrochloride as an internal standard (ISTD) by using HPLC-ESI-MS/ MS. Methods: The chromatographic separation was achieved with 20mM Ammonium acetate (pH-3.0):Methanol:Acetontrile (20:20:60,%v/v) using the TELOS LU C18 (2) 5μm, 100 x 4.6 mm. The total analysis time was 3 min and flow rate was set to 0.5 mL/min. Results: The mass transitions of Eravacycline dihydrochloride and Rolitetracycline hydrochloride obtained were m/z 632.5®84.3 and 436.2®84.3. The standard curve shows correlation coefficient (s) greater than 0.999 with a range of 15.00-120.00 pg/ml using the linear regression model. Conclusion: The method was suitable and conveniently applicable to pharmacokinetic and bioavailability studies for estimation of Eravacycline in biological matrices by HPLC-ESI-MS/MS.
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