Pharmaceutical Methods
Publishing Quality Research & Reviews
Pharmaceutical Methods
Publishing Quality Research & Reviews
Author(s): Darshan R. Telange, Arun T. Patil, Amol Tatode, and Bhushan Bhoyar
Introduction: Kaempferol (3, 5, 7-trihydroxy-2-(4-hydroxyphenyl)-4H-1-benzopyran-4-one) is a natural flavonoid belongs to a subcategory of flavonol family. The kaempferol – hydrogenated soy phosphatidylcholine (HSPC) complex was obtained by refluxing and freeze drying method. Ultra violet (UV) – visible spectrophotometric method has been developed for the determination of kaempferol in kaempferol – HSPC complex. Objective: A validated UV – visible spectrophotometric method for determination of kaempferol in Kaempferol – HSPC complex. Materials and Methods: The Kaempferol – HSPC complex (Phytosomes) were prepared by dissolving both kaempferol and HSPC in 1, 4 – dioxane for refluxing up to 2 h and freeze dried. The spectrophotometric detection of kaempferol was done at absorption maximum (λmax) of 365 nm and 265 nm using methanol as solvent. The developed method was validated as per ICH guidelines. Result: The kaempferol content in Kaempferol – HSPC complex was found to be 79.32% and 79.19% at 365 nm and 265 nm. Kaempferol demonstrated good linearity in concentration range of 2-12 μg/ml (r2 > 0.99) at 365 nm and 2-14 μg/ml (r2 > 0.99) at 265 nm. Precision and mean recoveries were found to be in the range of (%RSD 0.0957 and 0.0580) and (% RSD 0.1461 and 0.0959) and 99.70% and 91.85% at 365 nm and at 265 nm. Limit of detection and limit of quantification were found to be (0.015 μg/ml and 0.0191 μg/ml) and (0.0457 μg/ml and 0.0579 μg/ml) respectively. Conclusion: The developed method was found to be simple, specific, economic, reliable, accurate, precise, reproducible and used as a quality control tool for analysis of Kaempferol.
