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A validated HPTLC method for estimation of moxifloxacin hydrochloride in tablets

Submitted by webadmin on Thu, 10/13/2016 - 18:15
Pharmaceutical Methods,2010,1,1,54-56.
Published:October 2010
Type:Original Article

A validated HPTLC method for estimation of moxifloxacin hydrochloride in tablets

Vandana Dhillon, Alok Kumar Chaudhary1

Department of Pharmacy, IEC College of Engineering and Technology, Plot -4, Greater Noida, UP,

1Ranbaxy Research Laboratories, Sector-18, Plot-20, Gurgaon, Haryana - 122 001, India

Abstract:

A simple HPTLC method having high accuracy, precision and reproducibility was developed for the routine estimation of moxifloxacin hydrochloride in the tablets available in market and was validated for various parameters according to ICH guidelines. moxifloxacin hydrochloride was estimated at 292 nm by densitometry using Silica gel 60 F254 as stationary phase and a premix of methylene chloride: methanol: strong ammonia solution and acetonitrile (10:10:5:10) as mobile phase. Method was found linear in a range of 9-54 nanograms with a correlation coefficient >0.99. The regression equation was: AUC = 65.57 x (Amount in nanograms) + 163 (r2 = 0.9908).

HPTLC chromatogram of the constituted tablet placebo.