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Spectrofluorimetric estimation of salbutamol sulphate in different dosage forms by formation of inclusion complex with β-cyclodextrin

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Pharmaceutical Methods,2010,1,1,49-53.
Published:October 2010
Type:Original Article

Spectrofluorimetric estimation of salbutamol sulphate in different dosage forms by formation of inclusion complex with β-cyclodextrin

Harshit Narmadashankar Pandya, Hiren Harshadlal Berawala, Deepak Mohanlal Khatri, Priti Jignesh Mehta

Department of Pharmaceutical Analysis, Institute of Pharmacy, Nirma University, Sarkhej- Gandhinagar Highway, Ahmadabad - 382 481 Gujarat, India

Abstract:

A simple, precise, reproducible and accurate spectrofluorimetric method for estimation of Salbutamol sulphate (SAL) in bulk drug and various dosage forms has been developed. This method is based on formation of inclusion complex of SAL in β-cyclodextrin (BCD) which gives fluorescence at excitation wavelength of 279.6 nm and emission wavelength of 609.8 nm in water. Formation of inclusion complex of drug with BCD enhances fl uorescence intensity of drug leads to increased sensitivity. The developed method was validated according to ICH guidelines with respect to accuracy, precision, linearity, limit of detection, limit of quantification. Linearity was observed in the range of 4-20 μg/ ml with correlation coefficient of 0.9982. The simplicity of the method permitt ed rapid analysis suitable for routine control. The developed method was successfully applied for the estimation of SAL in diff erent marketed dosage forms like tablets, syrup and aerosol.

Spectrofluorimetric spectra of SAL