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HPLC for Peptides and Proteins: Principles, Methods and Applications

Submitted by webadmin on Tue, 10/11/2016 - 15:28
Pharmaceutical Methods,2017,8,1,01-06.
Published:October 2016
Type:Review Article

HPLC for Peptides and Proteins: Principles, Methods and Applications

Gaurav Pratap Singh Jadaun*, Shruti Dixit, Vandana Saklani, Sanjay Mendiratta, Renu Jain, Surinder Singh

Recombinant Product Laboratory, National Institute of Biologicals, (Ministry of Health and Family Welfare, Government of India), A-32, Sector-62, Institutional Area, Noida-201309, INDIA.


Peptides and proteins are large biomolecules composed of long chains of L -amino acids and plays key functions in the living organisms. A variety of techniques are used to characterize these biomolecules and, among the techniques used, high performance liquid chromatography (HPLC) has been extensively employed both for the preparative as well as the analytical characterization of peptides and proteins. Various HPLC techniques developed for the separation of proteins rely on the differences in the adsorption characteristics, surface charge, ligand specificity, and molecular size of protein molecules. Advancements made in these HPLC techniques have contributed immensely to the development of peptides and proteins based pharmaceuticals. Present article summarizes the principle, methods, and applications of the most common HPLC techniques used in the field of peptides and proteins.

A typical chromatographic procedure.