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Development and Validation of Few UV Spectrophotometric Methods for the determination of Valganciclovir in Bulk and Pharmaceutical Dosage Form

Submitted by webadmin on Fri, 08/03/2018 - 12:23
Pharmaceutical Methods,2018,9,2,64-68.
Published:August 2018
Type:Original Article

Development and Validation of Few UV Spectrophotometric Methods for the determination of Valganciclovir in Bulk and Pharmaceutical Dosage Form

Sumanta Mondal1*, Goluguri Sunil Reddy1, Prasenjit Mondal2, Vadlapati Sheeba Prathyusha1, Aishwarya P Nair1, Syed Tazib Rahaman1

1GITAM Institute of Pharmacy, GITAM (Deemed to be University), Visakhapatnam, Andhra Pradesh-530045, INDIA.

2Vaageswari college of Pharmacy, Ramakrishna colony, Karimnagar, Telangana. 505481 INDIA.

Abstract:

Background: The present research paper described about the optimization of various zero order and first order UV spectrophotometric method using different buffers. Validation study was performed to develop a simple, sensitive, rapid, accurate and economical Ultra Violet spectrophotometric method for the estimation of Valganciclovir. Methods: UV 1800 double beam UV Visible Spectrophotometer with a pair of 10mm path length matched quartz cells were used for the study. Method A (Water), Method B (phosphate buffer pH2), Method C (Phosphate buffer pH4) and Method D (phosphate buffer pH5) were developed for estimation of Valganciclovir by zero-order and first-order derivative. Results: Linearity was carried out in the concentration range of 5-60μg/ml and the correlation coefficient were found to be 0.999. The percentage recoveries were found to be 98-102%. The relative standard deviation was found to be <2%. The LOD and LOQ were found to be 0.3241 μg/ml and 0.8227 μg/ml respectively. Conclusion: Hence, the methods were validated according to ICH guidelines and can be adopted for the routine analysis of Valganciclovir in pure and tablet dosage form.

Pictorial Abstract