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RP-UPLC method development and validation for the simultaneous estimation of ibuprofen and famotidine in pharmaceutical dosage form

Submitted by webadmin on Wed, 10/26/2016 - 11:29
Pharmaceutical Methods,2012,3,2,57-61.
Published:December 2012
Type:Original Article

RP-UPLC method development and validation for the simultaneous estimation of ibuprofen and famotidine in pharmaceutical dosage form

Yarram Ramakoti Reddy, Kakumani Kishore Kumar, MRP Reddy1, K Mukkanti

Department of Chemical Science and Technology, Institute of science and Technology, Jawaharlal Nehru Technological University, Hyderabad,

1Centre for Meterials for Electronics Technology IDA, HCL post, Cherlapally, Hyderabad, India

Abstract:

Aim and Backrgound: A stability-indicating LC method was developed for the simultaneous determination of Ibuprofen and Famotidine in pharmaceutical dosage forms. Materials and Methods: The chromatographic separation was achieved on Acquity UPLC BEH C-18,50 mm x 2.1 mm and 1.7 μm column with gradient elution. The mobile phase A contains a mixture of 50 mM sodium acetate buffer (pH 5.5): methanol (85:15, v/v), and the mobile phase B contains a mixture of 50 mM sodium acetate buffer (pH 5.5): methanol (25:75, v/v). The flow rate was 0.3 mL min-1, and the detection wavelength was 260 nm. Results: The limit of detection for Ibuprofen and Famotidine was 1.6 and 1.2 μg mL-1, respectively. The limit of quantification (LOQ) for Ibuprofen and Famotidine was 5.1 and 4.3 μg mL-1, respectively. Conclusion: This method was validated for accuracy, precision, and linearity. The method was also found to be stability indicating.