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Spectrophotometric methods for simultaneous estimation of rabeprazole sodium and aceclofenac from the combined capsule dosage form

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Pharmaceutical Methods,2011,2,3,193-197.
Published:July 2011
Type:Symposium - HPLC

Spectrophotometric methods for simultaneous estimation of rabeprazole sodium and aceclofenac from the combined capsule dosage form

Ramesh L. Sawant, Sanket D. Hadawale, Ganesh K. Dhikale, Charusheela A. Bansode, Pravin S. Tajane

Department of Pharmaceutical Chemistry and PG Studies, P.D.V.V.P.F’s College of Pharmacy, Vilad Ghat, Ahmednagar, India

Abstract:

Introduction: The present work aimed to develop and validate spectrophotometric methods for simultaneous estimation of rabeprazole sodium and aceclofenac in a pure and capsule dosage form. Materials and Methods: Method 1 is based on solving a simultaneous equation. Absorbances of rabeprazole sodium and aceclofenac were measured at their respective absorbance maximas (λmax) of 283 and 276 nm. Method 2 is the Q-analysis or absorption ratio method. Absorbances were measured at 256 nm (isosbestic point) and 276 nm (λmax of aceclofenac). Methods are validated according to ICH guidelines. Results: A linearity range for rabeprazole sodium and aceclofenac is 10–60 μg/ml at respective selected wavelengths. The coefficient of correlation for rabeprazole at 283 nm and for aceclofenac at 276 nm is 0.9981 and 0.9997, respectively. A percentage estimation of rabeprazole sodium and aceclofenac from the capsule dosage form by method 1 is 100.22 and 99.96 and by method 2 is 99.99 and 100.05, respectively, with a standard deviation less than 2. Conclusion: The proposed methods are simple, rapid, and validated and can be used successfully for routine simultaneous estimation of rabeprazole sodium and aceclofenac in a pure and capsule dosage form.