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HPTLC Method Development and Validation of Cilnidipine and Metoprolol Succinate in Combined Dosage Form

Submitted by webadmin on Tue, 10/18/2016 - 12:29
Pharmaceutical Methods,2016,7,1,28-34.
Published:January 2016
Type:Original Article

HPTLC Method Development and Validation of Cilnidipine and Metoprolol Succinate in Combined Dosage Form

Dhwani Desai1*, Nirmal Vashi2, Hitesh Dalvadi2, Shuchi Desai1, Madhuri Hinge1

1Department of Quality Assurance, Rofel Shri G.M. Bilakhia, College of Pharmacy, Vipa, INDIA.

2Department of Pharmaceutics, Rofel Shri G.M. Bilakhia, College of Pharmacy, Vipa, INDIA.

Abstract:

Introduction and Objectives: The present work involves development and validation of HPTLC method for simultaneous estimation of Cilnidipine and Metoprolol Succinate in their combined tablet dosage form. Method: In HPTLC method, Silica Gel G60 F254 TLC plate as the stationary phase and a mobile phase of Toluene: Chloroform: Methanol: Glacial acetic acid (45: 25: 25: 5 v/v/v/v) was used to resolve CIL and METO. CIL and METO were quantified at 231 nm. The proposed method weas validated according to International Conference on Harmonization. Result and Discussion: Two well-separated and sharp peak for CIL and METO were obtained at Rf values of 0.70 ± 0.01 and 0.34 ± 0.005 respectively. The linearity range obtained for HPTLC method were 100-500 ng/spot and 500-2500 ng/spot for CIL and METO respectively. Conclusion: Method validation was found to be accurate, specific and precise.The developed method was successfully applied for estimation of CIL and METO in combined tablet formulation.

3-D chromatogram of CIL and METO showing linearity