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Spectrophotometric estimation of tamsulosin hydrochloride by acid-dye method

Submitted by webadmin on Mon, 10/17/2016 - 16:22
Pharmaceutical Methods,2011,2,1,53-60.
Published:January 2011
Type:Original Article

Spectrophotometric estimation of tamsulosin hydrochloride by acid-dye method

Alankar Shrivastava, Prachi Saxena, Vipin B. Gupta1

Pharmaceutical Analysis Department, 1BRNSS Group of Institutions, B.R. Nahata College of Pharmacy, Mhow-Neemuch Road, Mandsaur, Madhya Pradesh, India

Abstract:

A new spectrophotometric method for the estimation of tamsulosin hydrochloride in pharmaceutical dosage forms has been developed and validated. The method is based on reaction between drug and bromophenol blue and complex was measured at 421 nm. The slope, intercept and correlation coefficient was found to be 0.054, -0.020 and 0.999, respectively. Method was validated in terms of specificity, linearity, range, precision and accuracy. The developed method can be used to determine drug in both tablet and capsule formulations. Reaction was optimized using three parameters i.e., concentration of the dye, pH of the buffer, volume of the buffer and shaking time. Maximum stability of the chromophore was achieved by using pH 2 and 2 ml volume of buffer. Shaking time kept was 2 min and concentration of the dye used was 2 ml of 0.05% w/v solution. Method was validated in terms of linearity, precision, range, accuracy, LOD and LOQ and stochiometry of the method was also established using Mole ratio and Job’s method of continuous variation. The dye benzonoid form (blue color) of dye ionized into quinonoid form (purple color) in presence of buffer and reacts with protonated form of drug in 1:1 ratio and forms an ion-pair complex (yellow color).

Proposed mechanism of reaction of TAM and BPB by proposed method