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Simultaneous estimation of amlodipine besylate and nebivolol hydrochloride in tablet dosage forms by reverse phase-highperformance liquid chromatographic using ultraviolet detection

Submitted by webadmin on Mon, 10/17/2016 - 15:38
Pharmaceutical Methods,2011,2,1,9-14.
Published:January 2011
Type:Original Article

Simultaneous estimation of amlodipine besylate and nebivolol hydrochloride in tablet dosage forms by reverse phase-highperformance liquid chromatographic using ultraviolet detection

Deepak Sharma, Anurekha Jain, Alankar Shrivastava

Department of Pharmaceutical Analysis, B.R. Nahata College of Pharmacy, Mhow-Neemuch Road, Mandsaur, (M.P)-458 001, India

Abstract:

Background: The present study aimed to develop and validate the simultaneous estimation of amlodipine and nebivolol in tablet dosage forms. Materials and Methods: An isocratic reversed phase high-performance liquid chromatographic (HPLC) method with ultraviolet detection at 268 nm has been developed for the determination of amlodipine besylate (ADB) and nebivolol hydrochloride in dosage formulation. Results: Good chromatographic separation was achieved by using a stainless steel analytical column, the Lichrospher ODS RP-18 column (250 × 4 mm), particle size 5 μm. The system was operated at ambient temperature (25 ± 2°C) using a mobile phase consisting of acetonitrile (ACN) and a phosphate buffer (pH 3.0), mixed in a ratio of 40 : 60 at a flow rate of 0.8 ml/minute. The slope, intercept, and correlation coefficient were found to be 8818.2, - 18159, and 0.9993 for amlodipine and 9048.7, 108595, and 0.9998 for nebivolol, respectively. The proposed method was validated for its specificity, linearity, accuracy, and precision. Conclusion: The method was found to be suitable for the quality control of amlodipine besylate and nebivolol hydrochloride simultaneously in a bulk drug as well as in a formulation.

Overlay spectra of the various concentration level chromatograms of the two drugs